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1.
Acta Pharmaceutica Sinica ; (12): 1258-1261, 2009.
Article in English | WPRIM | ID: wpr-344098

ABSTRACT

Two erythrodiol triterpene fatty esters, 3beta-dodecanoyl erythrodiol (1) and 3beta-tetradecanoyl erythrodiol (2), were isolated from Scorzonera mongolica. Their structures were elucidated on the basis of IR, MS and extensive 2D NMR spectroscopic analysis. Compound 1 was identified to be a new compound and 2 was confirmed to be a new natural compound. Their antitumor effects in vitro were evaluated with MTT and SRB assays, but compounds 1 and 2 only showed moderate cytotoxicities on A-549 cell line.


Subject(s)
Animals , Humans , Mice , Antineoplastic Agents, Phytogenic , Chemistry , Pharmacology , Carcinoma, Hepatocellular , Pathology , Cell Line, Tumor , Drugs, Chinese Herbal , Chemistry , Pharmacology , Leukemia P388 , Pathology , Liver Neoplasms , Pathology , Lung Neoplasms , Pathology , Magnetic Resonance Spectroscopy , Molecular Structure , Plants, Medicinal , Chemistry , Scorzonera , Chemistry , Triterpenes , Chemistry , Pharmacology
2.
Chinese Journal of Pediatrics ; (12): 513-517, 2006.
Article in Chinese | WPRIM | ID: wpr-278668

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the isolation and expansion of mesenchymal stem cells (MSCs) from human umbilical cord Wharton's jelly and their biological identities, and explore the possibility of inducing human umbilical cord-derived MSCs to differentiate into neurocyte-like cells.</p><p><b>METHODS</b>The growth and proliferative abilities of human umbilical cord-derived MSCs were observed, and their immunophenotypes were determined by flow cytometry. Salvia miltiorrhiza and beta-sulfhydryl alcohol were adopted to induce the cells to differentiate. The differentiated and undifferentiated cells were identified with immunocytochemistry. The pleiotrophin and nestin genes were measured by RT-PCR.</p><p><b>RESULTS</b>A population of human umbilical cord-derived MSCs were isolated from human umbilical Wharton's jelly; they were processed to obtain a fibroblast-like population of cells and could be maintained in vitro for extended periods with stable population doubling, and they were expanded as undifferentiated cells in culture for more than 10 passages, indicating their proliferative capacity. The human umbilical cord-derived MSCs were positive for CD(29), CD(44), CD(59), CD(105), but negative or weakly expressed the markers of hematopoietic cells such as CD(14), CD(33), CD(34), CD(28), CD(45) and CD(117). The important GVHD correlation markers were negative or weakly expressed, including CD(80) (B7-1), CD(86) (B7-2), CD(40) and CD(40L). Salvia miltiorrhiza beta-sulfhydryl alcohol could induce the MSCs to express nestin, a marker of neuronal precursor stem cells at early stage of differentiation. Later, they exhibited neural phenotypes, expressing beta-tubulin III and neurofilament (NF) and glial fibrillary acidic protein (GFAP). It was confirmed by RT-PCR that the MSCs could express pleiotrophin either before or after the induction of salvia miltiorrhiza, furthermore, after the induction the expression was markedly enhanced and the nestin gene was also expressed.</p><p><b>CONCLUSION</b>The human MSCs could be isolated from human umbilical cord Wharton's jelly, and it was easy to propagate these MSCs. The negative GVHD correlated markers might result from the fact that MSCs had no HLA barrier, which may suggest potential clinical significance. The MSCs are capable of differentiating into neurocyte-like cells and they may represent an alternative stem cell source for CNS cells transplantation.</p>


Subject(s)
Female , Humans , Infant, Newborn , Male , Pregnancy , Antigens, CD , Allergy and Immunology , Carrier Proteins , Genetics , Cell Differentiation , Physiology , Cells, Cultured , Cytokines , Genetics , Flow Cytometry , Glial Fibrillary Acidic Protein , Metabolism , Immunohistochemistry , Intermediate Filament Proteins , Genetics , Mesenchymal Stem Cells , Allergy and Immunology , Metabolism , Physiology , Nerve Tissue Proteins , Genetics , Nestin , Neurofilament Proteins , Metabolism , Neurons , Metabolism , Physiology , Reverse Transcriptase Polymerase Chain Reaction , Tubulin , Metabolism , Umbilical Cord , Cell Biology
3.
Biomedical and Environmental Sciences ; (12): 329-335, 2006.
Article in English | WPRIM | ID: wpr-229680

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the differentiation of bone marrow stromal cells (BMSC) into neuron-like cells and to explore their potential use for neural transplantation.</p><p><b>METHODS</b>BMSC from rats and adult humans were cultured in serum-containing media. Salvia miltiorrhiza was used to induce human BMSC (hBMSC) to differentiate. BMSC were identified with immunocytochemistry. Semi-quantitative RT-PCR was used to examine mRNA expression of neurofilamentl (NF1), nestin and neuron-specific enolase (NSE) in rat BMSC (rBMSC). Rat BMSC labelled by Hoschst33258 were transplanted into striatum of rats to trace migration and distribution.</p><p><b>RESULTS</b>rBMSC expressed NSE, NF1 and nestin mRNA, and NF1 mRNA and expression was increased with induction of Salvia miltiorrhiza. A small number of hBMSC were stained by anti-nestin, anti-GFAP and anti-S100. Salvia miltiorrhiza could induce hBMSC to differentiate into neuron-like cells. Some differentiated neuron-like cells, that expressed NSE, beta-tubulin and NF-200, showed typical neuron morphology, but some neuron-like cells also expressed alpha smooth muscle protein, making their neuron identification complicated. rBMSC could migrate and adapted in the host brains after being transplanted.</p><p><b>CONCLUSION</b>Bone marrow stromal cells could express phenotypes of neurons, and Salvia miltiorrhiza could induce hBMSC to differentiate into neuron-like cells. If BMSC could be converted into neurons instead of mesenchymal derivatives, they would be an abundant and accessible cellular source to treat a variety of neurological diseases.</p>


Subject(s)
Animals , Humans , Male , Rats , Bone Marrow Transplantation , Brain , Cell Biology , Cell Differentiation , Cells, Cultured , Drugs, Chinese Herbal , Pharmacology , Neurons , Cell Biology , Plant Extracts , Rats, Sprague-Dawley , Salvia miltiorrhiza , Stromal Cells , Cell Biology , Transplantation
4.
Chinese Journal of Traumatology ; (6): 77-80, 2005.
Article in English | WPRIM | ID: wpr-338639

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the differentiative capability of adult human bone marrow mesenchymal stem cells (BMSCs) into Schwann-like cells.</p><p><b>METHODS</b>Bone marrows were aspirated from healthy donors and mononuclear cells were separated by Percoll lymphocytes separation liquid (1.073 g/ml) with centrifugation, cells were cultured in DMEM/F12 (1:1) medium containing 10% fetal bovine serum (FBS), 20 ng/ml epidermal growth factor (EGF) and 20 ng/ml basic fibroblast growth factor (bFGF). Cells of passage 1 were identified with immunocytochemistry.</p><p><b>RESULTS</b>Mononuclear cells separated by Percoll's were passaged 10 times by trypsin/ethylenediaminetetraacetic acid (EDTA) digestion in 40 days, and BMSCs increased about 6x10(7) times in this short period. Immunohistochemistry identified that BMSCs were CD34- and CD31-, but they expressed neuron specific enolase; 0.01%-0.02% of total cells expressed nestin, the marker for neural progenitor cells; 40%-50% cells stained heavily by neurofilament 200; and no glial fibrillary acidic protein (GFAP) positive cells were identified; S100 expression was detected among 0.1%-0.2% cells.</p><p><b>CONCLUSIONS</b>Bone marrow contains the stem cells with the ability of differentiating into Schwann-like cells, which may represent an alternative stem cell sources for neural transplantation.</p>


Subject(s)
Adult , Humans , Bone Marrow Cells , Cell Biology , Metabolism , Cell Differentiation , Physiology , Cell Proliferation , Immunohistochemistry , Intermediate Filament Proteins , Metabolism , Mesenchymal Stem Cells , Cell Biology , Metabolism , Nerve Tissue Proteins , Metabolism , Nestin , Neurofilament Proteins , Metabolism , Phosphopyruvate Hydratase , Metabolism , S100 Proteins , Metabolism , Schwann Cells , Cell Biology
5.
Chinese Medical Journal ; (24): 1987-1993, 2005.
Article in English | WPRIM | ID: wpr-282834

ABSTRACT

<p><b>BACKGROUND</b>The two most basic properties of mesenchymal stem cells (MSCs) are the capacities to self-renew indefinitely and differentiate into multiple cells and tissue types. The cells from human umbilical cord Wharton's Jelly have properties of MSCs and represent a rich source of primitive cells. This study was conducted to explore the possibility of inducing human umbilical cord Wharton's Jelly-derived MSCs to differentiate into nerve-like cells.</p><p><b>METHODS</b>MSCs were cultured from the Wharton's Jelly taken from human umbilical cord of babies delivered after full-term normal labor. Salvia miltiorrhiza and beta-mercaptoethanol were used to induce the human umbilical cord-derived MSCs to differentiate. The expression of neural protein markers was shown by immunocytochemistry. The induction process was monitored by phase contrast microscopy, electron microscopy (EM), and laser scanning confocal microscopy (LSCM). The pleiotrophin and nestin genes were measured by reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>MSCs in the Wharton's Jelly were easily attainable and could be maintained and expanded in culture. They were positive for markers of MSCs, but negative for markers of hematopoietic cells and graft-versus-host disease (GVHD)-related cells. Treatment with Salvia miltiorrhiza caused Wharton's Jelly cells to undergo profound morphological changes. The induced MSCs developed rounded cell bodies with multiple neurite-like extensions. Eventually they developed processes that formed networks reminiscent of primary cultures of neurons. Salvia miltiorrhiza and beta-mercaptoethanol also induced MSCs to express nestin, beta-tubulinIII, neurofilament (NF) and glial fibrillary acidic protein (GFAP). It was confirmed by RT-PCR that MSCs could express pleiotrophin both before and after induction by Salvia miltiorrhiza. The expression was markedly enhanced after induction and the nestin gene was also expressed.</p><p><b>CONCLUSIONS</b>MSCs could be isolated from human umbilical cord Wharton's Jelly. They were capable of differentiating into nerve-like cells using Salvia miltiorrhiza or beta-mercaptoethanol. The induced MSCs not only underwent morphologic changes, but also expressed the neuron-related genes and neuronal cell markers. They may represent an alternative source of stem cells for central nervous system cell transplantation.</p>


Subject(s)
Humans , Cell Differentiation , Cells, Cultured , Glial Fibrillary Acidic Protein , Immunohistochemistry , Mesenchymal Stem Cells , Cell Biology , Neurofilament Proteins , Neurons , Cell Biology , Reverse Transcriptase Polymerase Chain Reaction , Tubulin , Umbilical Cord , Cell Biology
6.
Chinese Medical Journal ; (24): 425-429, 2004.
Article in English | WPRIM | ID: wpr-346658

ABSTRACT

<p><b>BACKGROUND</b>Adipose tissue-derived stromal cells (ADSCs) can be greatly expanded in vitro, and induced to differentiate into multiple mesenchymal cell types, including osteogenic, chondrogenic, myogenic, and adipogenic cells. This study was designed to investigate the possibility of ADSCs differentiating into neurons.</p><p><b>METHODS</b>Adipose tissue from rats was digested with collagenase, and adherent stromal cells were cultured. A medium containing a low concentration of fetal bovine serum was adopted to induce the cells to differentiate. ADSCs were identified by immunocytochemistry, and semi-quantitative RT-PCR was applied to detect mRNA expression of neurofilament 1 (NF1), nestin, and neuron-specific enolase (NSE).</p><p><b>RESULTS</b>Nestin-positive cells were found occasionally among ADSCs. ADSCs were found to express NSE mRNA and nestin mRNA, but not NF1 mRNA. ADSCs could differentiate into neuron-like cells in a medium composed of a low concentration of fetal bovine serum, and these differentiated cells displayed complicated neuron-like morphologies.</p><p><b>CONCLUSIONS</b>The data support the hypothesis that adipose tissue contains stem cells capable of differentiating into neurons. These stem cells can overcome their mesenchymal commitment, and may represent an alternative autologous stem cell source for CNS cell transplantation.</p>


Subject(s)
Animals , Rats , Adipose Tissue , Cell Biology , Cell Differentiation , Physiology , Cells, Cultured , Immunohistochemistry , Intermediate Filament Proteins , Nerve Tissue Proteins , Nestin , Neurofilament Proteins , Neurons , Cell Biology , Phenotype , Phosphopyruvate Hydratase
7.
Chinese Journal of Traumatology ; (6): 91-95, 2004.
Article in English | WPRIM | ID: wpr-270245

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the culture method of skin-derived precursors (SKPs) and to explore a new cell source for cell transplantation of central nervous system.</p><p><b>METHODS</b>Cells from skins of juvenile and adult mice were isolated and cultured in serum-free medium. A mechanical method was chosen to passage these cells and they were identified by the immunocytochemistry assay.</p><p><b>RESULTS</b>SKPs could be isolated from adult and neonatal skins. They could be maintained in vitro for long periods with stable proliferation, and expanded as undifferentiated cells in culture for more than 12 passages. About 50% of SKPs expressed nestin and majority of these cells expressed fibronectin when they were plated on polyornithine and laminin coated plates. About 5% cells showed neuronal differentiation and expressed neurofilament-M (NF-M) and NSE when SKPs were plated in serum-containing medium, and these cells could also differentiate into adipocytes and fibroblast-like cells.</p><p><b>CONCLUSIONS</b>The data support the hypothesis that adult skin contains stem cells capable of differentiating into neurons, adipocytes, and fibroblast-like cells. They may represent an alternative autologous stem cell source for CNS cell transplantation.</p>


Subject(s)
Animals , Mice , Adipocytes , Cell Differentiation , Physiology , Cells, Cultured , Immunohistochemistry , Mice, Inbred BALB C , Neurons , Skin , Cell Biology , Stem Cell Transplantation
8.
Chinese Journal of Traumatology ; (6): 341-345, 2003.
Article in English | WPRIM | ID: wpr-270300

ABSTRACT

<p><b>OBJECTIVE</b>To explore the culture conditions of human neural stem cells and to investigate the ultrastructure of neurospheres.</p><p><b>METHODS</b>The cells from the embryonic human cortices were mechanically dissociated. N2 medium was adapted to culture and expand the cells. The cells were identified by immunocytochemistry and EM was applied to examine the ultrastructure of neurospheres.</p><p><b>RESULTS</b>The neural stem cells from human embryonic brains were successfully cultured and formed typical neurospheres in suspension, and most of the cells expressed vimentin, which was a marker for neural progenitor cells, and the cells could differentiate into neurons, astrocytes and oligodendrocytes. In vitro myelin formation in neurospheres were observed at an early stage of culture.</p><p><b>CONCLUSIONS</b>Human neural stem cells can be cultured from embryonic brains, can form the typical neurospheres in suspension in vitro and have the ability of myelinating, and may be potential source for transplantation in treating myelin disorders.</p>


Subject(s)
Female , Humans , Male , Brain , Cell Biology , Cells, Cultured , Culture Media , Immunohistochemistry , Microscopy, Electron , Myelin Sheath , Pathology , Neurons , Cell Biology , Pathology , Sensitivity and Specificity , Stem Cell Transplantation , Stem Cells , Physiology
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